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nearly as much as the enzyme concentration. The effects of varying temperature are the next part of the experiment. Temperature can be a denaturing factor at the wrong levels, as shown by the peak in the graph. (I explained earlier that the dip was cause by experimental error.) Before the peak is a constant curve, and after the peak, the curve should be a nearly constant negatively sloped curve. The Pastinaca parsnip extract actually reaches its peak, optimum temperature, at 22 degrees for guaiacol oxidation. After that, it appears as if the enzyme becomes denatured and loses efficiency. Boiling the enzyme completely denatures it and causes it to have no effect. As the kinetic energy increased, the randomness of the particles disrupted the enzyme’s ability to facilitate the reaction. The effects of varying pH are very similar to the effects of varying temperature. There is an optimum pH for the guaiacol oxidation, at which the maximum absorbance rate can be observed. Before and after this, the enzyme can be considered denatured, because of the lack of efficiency from the enzyme. The optimum pH appears to be just before 7, which a slightly acidic condition. I concluded that the turnip would actually grow best in a condition of 22 degrees and a soil pH of about 6.3. ConclusionI. Enzyme, turnip peroxidase, concentration is directly related to the rate of reaction. As the concentration increases, the rate increases; and when the concentration decreases, the rate also decreases.II. Substrate, peroxide, concentration has minimal effects on the guaiacol oxidation, however the baseline concentration appears to be the preferred concentration of substrate. III. As the temperature increases, the results show a steady increase, a peak, and a steady decrease in the rate of reaction until the peroxidase becomes inactive. Once the enzyme reaches its optimum temperature, which appears to be 22 degrees, the rate of oxidation will decline afterwards. IV. Varying pH produces similar effect to varying temperatures, except that the optimum pH occurs at around 6.3 on the pH scale. V. Peroxidase is important because it converts H2O2 to H2O. The H2O2 was produced by oxygen metabolism and must be removed because it is highly toxic.Turnips would most likely prefer conditions in which the soil has a slightly acidic pH and a temperature near that of a normal room.